Comments for The RNA Underworld

Comment on While in Lexington … by sirdm

sirdm — Fri, 03 May 2013 17:53:28 +0000

Prof. Hunt, I went to the first session and the second session and did a write-up on the talks (which I am willing to share with you if you are interested). I found this webpage while searching for notes from Dr. Krishnamurthy’s talk, which I was unable to attend due to a scheduling conflict. If you went to that one and have notes you’re willing to share it would be great! sdmo228@g.uky.edu Thank you!

Comment on The Poly(A) Tail by Sara

Sara — Mon, 15 Apr 2013 06:11:36 +0000

Why is it a poly A tail ? why not poly C or U ???

Comment on The Poly(A) Tail by Francisco Guillermo

Francisco Guillermo — Wed, 10 Apr 2013 03:01:25 +0000

In eukaryotic cells, can there be a transcribed mRNA without a poly A tail, and in such a case could that mRNA be able to be transported from the nucleo to the cytoplasm and then be translated? I know that the poly A is part of the processing mechanism of the mRNA, and that its need it to be transported trough the nucleous membrane, as well as for translation, but I was searching for any exeptions. Well if you know of an exeption I´d really appreciate it if you could help me out.

Thanks.

Comment on December 6, 1941 by elmore

elmore — Fri, 08 Mar 2013 13:44:45 +0000

Amazing, there is the truth about WW2 and then there is Tom Brokaw’s stupid book. This is a perfect book for people of questionable intelligence that haven’t a clue about actual history and are too lazy too examine the historical records to obtain the truth about World War 2 or to put it more bluntly it’s a feel good book for stupid people. A more accurate description of the time can be found from an old TV documentary that aired in the US in 1975 called THE WORLD AT WAR. This British made 30 part series of one hour shows took you from the political beginning of the Nazi’s during the great depression right through to the bitter end of the Axis forces. This film came out in the cold war years and was surprisingly accurate considering the self serving propaganda of the former allies at the time. I was only 28 years old back then when I watched the entire series and I thought, they never taught this in school.

Comment on On the 47th day … by Arthur Hunt

Arthur Hunt — Sat, 23 Feb 2013 02:35:17 +0000

On the brighter side, seeing the whole of Quadrophenia live was a blast.

Comment on On the 47th day … by Arthur Hunt

Arthur Hunt — Sat, 23 Feb 2013 02:32:01 +0000

Yeah. All different sorts. Two of the new NSF IOS preproposals, a full NSF proposal, a USDA proposal, and one for a state program. The fact that NSF is moving to a once-per-year submission system has changed the long-range planning for new projects. One needs a 2+ year outlook now and a whole lot of patience.

Comment on On the 47th day … by Clint MacDonald

Clint MacDonald — Sun, 17 Feb 2013 20:33:06 +0000

Five grant proposals in a month-and-a-half. Whoa.

Comment on How bad … by ongenetics

ongenetics — Thu, 14 Feb 2013 14:07:43 +0000

Art, I first thought this was a picture of you, perhaps with a wig or something. There is a definite resemblance.

Comment on Next stop – Kansas! by Santanu

Santanu — Sun, 10 Feb 2013 04:59:13 +0000

All the best to Amy! I can not believe, she is a lady now! Your mom looks as young as I have seen her more than 1
7 years ago I guess!

Comment on How bad … by Clem

Clem — Fri, 04 Jan 2013 20:56:29 +0000

Oh now… be thankful your Mom cares enough. I will say it must be tough being temporarily out of the top 25.

Here’s wishing you and yours a Happy New Year. Now let’s get the Cats back in the top ten…

Comment on Backdrop and background …. by Balu

Balu — Tue, 01 Jan 2013 02:37:42 +0000

It is heartening to see a good amount of interest in these research findings.
Wish you all a Peaceful, Productive and Reflective New Year!

Comment on Backdrop and background …. by Arthur Hunt

Arthur Hunt — Wed, 05 Dec 2012 21:50:14 +0000

Hi Catherine,

To answer your questions:

1. We can indeed dissociate the calmodulin binding from the RNA binding. The 30M mutant you mention does not bind calmodulin, but it does bind RNA as well as the wild-type protein, as far as we can tell. This is good since it helps with the complementation experiments you suggest.

2. We are currently studying oxt6 lines that express wild-type CPSF30 as well the 30M mutant. Briefly, the 30M mutant does complement some of the mutant phenotypes but not others – a paper on this is in the works. We have some high throughput sequencing data for these plants, but we may need more data before we can say anything definitive about the polyadenylation phenotypes of the various lines.

Comment on Backdrop and background …. by bergounioux

bergounioux — Wed, 05 Dec 2012 09:07:07 +0000

Dr Hunt,
Thank you very much for your answer. Please could you dissociate the calmodulin binding to the RNA binding for the complementation? Is the 30M mutant able to complement oxt6?
Catherine

Comment on Backdrop and background …. by Arthur Hunt

Arthur Hunt — Tue, 04 Dec 2012 19:39:19 +0000

(Dr?) Bergounioux, Thanks for your interest in this essay and in the subject of the study. The gene that encodes CPSF30 in plants yields two different mRNAs. One in turn may be translated to yield the 250 amino acid CPSF30, while the other yields a larger (>650 amino acid) polypeptide. The smaller protein has been extensively studied, as the publication list at the end of the essay indicates. We don't know much about the larger protein. We are studying oxt6 plants that express different forms of CPSF30 (small, large, etc.) and will hopefully have an interesting story to tell in the future. More about the two CPSF30 mRNA isoforms in plants can be found in reference 2 and in a recent paper that I plan on discussing on this blog in the near future.

Comment on Backdrop and background …. by bergounioux

bergounioux — Tue, 04 Dec 2012 17:08:08 +0000

Dear Colleague,
CPF30 has a spliced form? This form is not mentioned in your manuscript? Could you differentiate the two forms in your study?
Thanks
C. Bergounioux